OK器械消毒液杀灭微生物效果CJ[100]与腐蚀性的试验观察

陈越英　顾健　吴小成　周士新　张晓东 　　提要　OK器械消毒液为含2%戊二醛的复方消毒剂。对枯草杆菌黑色变种芽胞作用3h，杀灭率为100%，作用10min可将HBsAg抗原性破坏。杀菌效果随温度升高而增强，随pH下降、悬液中含有机物、复方中去除亚硝酸钠而减弱。所试金属，除对铝有轻度腐蚀外均基本无腐蚀。存放于54～57℃ 14d，戊二醛浓度下降2.97%。 　　关键词　戊二醛复方消毒液　杀菌作用　HBsAg　大肠杆菌　金黄色葡萄球菌　细菌芽胞　枯草杆菌黑色变种　腐蚀性　稳定性 EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND CORROSIVENESS OF OK INSTRUMENTdISINFECTION SOLUTION Chen Yueying　Gu Jian　Wu Xiaocheng　Zhou Shixin　Zhang Xiaodong (Jiangsu Provincial Sanitary and Anti-epidemic Station,Nanjing 210009) Abstract　The OK Instrumentdisinfection Solution is a compounddisinfectant (pH 7.0) containing 2% glutaraldehyde,0.1% compound alcohol,0.4% sodium nitrite and surfactant. The killing rate of the spores of Bacillus subtilis var. niger in suspension exposed to thisdisinfectant for 3h was 100%. Exposure to thisdisinfectant for 3h was 100%. Exposure to thisdisinfectant for 10min coulddestroy the antigenicity ofhBsAg in 5% calf serum. The killing rate of both Escherichia coli Fsexposed to 100-folddilute solution of thisdisinfectant for 15min and Staphylococcus aureus exposed to the same solution for 30min was 100%. Its germicidal efficacy increased with increasing the temperature anddecreased withdecreasing pH,presence of organic substances and removal of sodium nitrite in the compounddisinfectant.It was slightly corrosive to aluminum and essentially non-corrosive to copper,stainless steel and carbon steel. The concentration of glutaraldehyde in its stock solutiondecreased by 2.97% after storing at 54～57℃ for 14days. Key words　compound glutaraldehydedisinfection solution　germicidal effect　HBsAg　Escherichia coli　Staphylococcus aureus　bacterial spore　Bacillus subtilis var. niger&corrosiveness　stability32 　　OK器械消毒液为含2%戊二醛、0.1%复合醇、0.4%亚硝酸钠与表面活性剂的中性（pH 7.0）复方消毒剂。在实验室对其杀灭微生物效果及对金属腐蚀性等进行了试验观察。现将结果报道如下。 　　1 方法 　　1.1 中和剂选择试验 　　试验菌为大肠杆菌（8099）与枯草杆菌黑色变种（ATCC 9372）芽胞。试验分组为：①消毒剂＋菌液；②（消毒剂＋菌液）＋中和剂；③（消毒剂＋中和剂）＋菌液；④ 中和剂＋菌液；⑤ 正常菌对照；⑥ 未接种菌的培养基对照。试验结果，当第6组不长菌，第3、4、5组组间菌数差不超过10%，第1组不长菌或长菌量远低于第2组，第2组长菌量超过100cfu/ml时，表明所用中和剂及其浓度适宜〔1〕。 　　1.2定量杀菌试验 　　试验采用悬液法。试验菌为枯草杆菌黑色变种芽胞、大肠杆菌（ATCC 25922）、金黄色葡萄球菌（ATCC 6538），均制成含1%蛋白胨的菌悬液。试验时，将0.5ml菌悬液与4.5ml消毒液（对照为磷酸盐缓冲液）混匀。作用至规定时间，取菌药混合液0.5ml，加入盛有4.5ml中和剂的试管中，混匀。中和作用10min，作活菌计数，计算杀灭率。 　　1.3乙型肝炎表面抗原破坏试验 　　试验用悬液法进行。试验时，以含5%小牛血清的磷酸盐缓冲液将纯化乙型肝炎表面抗原（HBsAg）稀释至浓度为10&mu;g/ml后，取0.1ml与0.4ml消毒液混匀。作用至规定时间，再加0.5ml中和剂。中和作用10min，用ELISA法测OD值。同时设HBsAg阳性与阴性对照，以试验组OD值（S）与阴性对照组OD值（N）的比值（S/N）< 2.1为HBsAg抗原性破坏。 　　1.4稳定性试验 　　将消毒液原包装置54～57℃水浴中2周。于放置前、后，分别取样测戊二醛含量，计算下降率。同时，对放于室温下70d的消毒液进行对枯草杆菌黑色变种芽胞杀灭效果检测。 　　1.5金属腐蚀试验 　　将不锈钢、碳钢、铝、铜等金属制成直径2.4cm、厚0.1cm的圆片。试验时，每种金属取3片，将不同金属分别经该消毒液浸泡72h，于浸泡前、后称重，取3片平均值计算腐蚀速率（R）〔2〕。 　　2 结果 　　2.1 中和剂试验结果 　　试验以含1.0%甘氨酸、0.5%吐温80的磷酸盐缓冲液中和含2%戊二醛的OK器械消毒液（对细菌芽胞）或含0.02%戊二醛的OK器械消毒液（对大肠杆菌），结果均达到所定指标（表1）。 表1 OK器械消毒液中和试验结果 Table 1　Results of neutralization test for OK Instrumentdisinfection Solution 组别 Group 不同菌平均菌数(cfu／ml) Average bacterial count (cfu／ml) 枯草杆菌黑色变种芽胞 Spores of B. subtilis var. niger 大肠杆菌 E.coli 消毒剂+菌液 Disinfectant+bacterial suspension 0 0 (消毒剂+菌液)+中和剂 (Disinfectant+bacterial suspension)+neutralizer 330 1750 (消毒剂+中和剂)+菌液 (Disinfectant+neutralizer)+bacterial suspension 1220000 2950000 中和剂+菌液 Neutralizer+bacterial suspension 1210000 2970000 正常菌对照 Normal bacteria control 1220000 2940000 未接种菌的培养基对照 Uninoculated culture medium control 0 0 注: 试验温度为22℃。作用于细菌芽胞的OK器械消毒液含2%戊二醛，作用于大肠杆菌者为OK器械消毒液的1∶100稀释液。加细菌芽胞与大肠杆菌后作用时间分别为2h与15min。加中和剂后作用10min。结果为3次试验平均值。 Note:The temperature was 22℃. The OK Instrumentdisinfection solution for killing bacterial spores contained 2% glutaraldehyde and that for killing E.coli was 1∶100dilute solution of OK Instrumentdisinfection solution. The exposure time of bacterial spores and E.coli to thedisinfectant was 2h and 15min respectively. The neutralizing time was 10min. The results are means of 3 tests.     更多相关文档请点击>>效果(1000)消毒(491)试验(780)OK(4)中和(62)was(39)杆菌(412)作用(2694)观察(3338)